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As the first VEGF-targeted agent approved by FDA, bevacizumab, is used since February 2004, for the treatment of patients suffering from metastatic (m) CRC in combination with the standard chemotherapy treatment (as first-line treatment) [51]

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Subsequently, wash all the wells three times with 100 L of Hanks Balanced Salt Solution (HBSS)

This method was selected as it is rapid, reliable, and ethically accepted when performed under deep anaesthesia

p = 0.012)